Bionut Biology Website

 

 





Lab Page


CURRENT/MOST RECENT
* Not all laboratory assignments have items posted on this page.
   We do many labs and only a select few usually end up posted here.

 

Gel Electrophoresis Lab

Above is a good representation of the results this lab usually yields.  In each of the first three lanes,  notice
the individual colored bands (red, yellow, and blue respectively).  I have provided lines (the same color of 
each band in lanes 1-3) to show the furthest distance each band traveled.  Lanes 4-6 each contain the 
unknown mixtures (A, B, & C respectively).  The unknown mixtures each contain 2-3 of the known dyes.
In lane 4, unknown Mixture A contained all three dyes.  Unknown mixtures B & C (lanes 5 & 6 respectively)
each contained two of the known dyes.  Although two of the bands did not separate fully in lanes 4 & 5 
(unknown mixtures A & B), you can use the colored lines I provided to infer the furthest point traveled by 
each of the different colored bands.  

A metric ruler is provided at the left of the gel.  Measurements should be in millimeters.
(Known dye #1 traveled 26 mm)

 

PAST
Caterpillar Inquiry Lab 2005

For this lab, students were placed into groups and given a caterpillar egg.  It was the first "lab specimen" they viewed using the stereomicroscopes.  In just a few days, caterpillars emerged from the eggs and the students  observed their development through the larval/catepillar stage to pupa stage and, in half of the groups, to the adult/butterfly stage (the other half seems to be "overwintering").  The students were given very few instructions as far as how to collect data.  My only requirement (besides careful handling and proper care) was that they draw at least one picture of their specimen every class day.  The students were able to decide on their data collection method and format, etc.  Just glancing around during lab time I saw many wonderful drawings and observations!  I had always wanted to try to do a caterpillar/butterfly lab in the spring.  But by the time eggs are being deposited, school is almost out or sometimes already over.  This fall, I had an unexpected and large amount of eggs deposited on the "host plants" I am using for my Entomology graduate research project.  The eggs had to be removed from my research plants and I decided to take them in for the students to see.  At first, I was a little uneasy about just giving the students the eggs and saying "do something" with these, with no real structure.  It has turned out to be a WONDERFUL decision and a great learning experience for me, too.  The students really liked watching the caterpillars grow and the lab had (and still has) them asking a lot of questions -- and that's exactly where science (and learning) begins!

   ---------------------------------------------------------------------------------

   
These little "guys" had me quite busy on Friday evening and Saturday morning since they all decided to emerge at about the same time and on the weekend!  I wanted you to be able to see how they looked right after they emerged from their eggs.  I did my best with the pictures and some are better than others.  I tried to take an overall and a close-up shot of each, but some didn't come out well and are not included.  Please take a look at other groups' caterpillars, as well as your own.  I especially like Black group # 5's picture that shows the empty egg shell and  #4 appears to be emerging from its egg (or has just emerged and is eating the egg shell?).  Do you know what the egg's outer covering is made out of?  For 2 pts added on to your lab, let me know the answer via E-mail (a link is provided at the bottom of this page).  I also like the picture of Red group #4 which shows the caterpillar chewing on the leaf (see the chewed pieces?).    

* For a closer, more detailed view of each picture, click on its thumbnail.

 

Emerged on Sept. 23, 2005

                   Black Group (Blocks 2A & 4B)

Black 1.JPG (361960 bytes)    Black 1 (upclose).JPG (315957 bytes)     Black 4.JPG (387884 bytes)    Black 4 (upclose).JPG (370235 bytes)    
       1               1                 4                4  

Black 5.JPG (374717 bytes)  Black 5 (2).JPG (322464 bytes)   Black 6.JPG (393348 bytes)   Black 6 (3).JPG (358121 bytes)  
       5               5              6               6              
*notice the empty egg shell


                   Blue Group (Blocks 3A & 4B)

Blue 1.JPG (373911 bytes)   Blue 1 (4).JPG (364503 bytes)    Blue 2.JPG (378248 bytes)   Blue 2 (4).JPG (367057 bytes)  
       1               1                2                2   

              


      Red Group (Blocks 1B & 2B)

Red 1.JPG (411013 bytes)  Red 1 (1).JPG (335512 bytes)  Red 3.JPG (370674 bytes)  Red 3 (3).JPG (351285 bytes)
       1                1              3                3

Red 4.JPG (392353 bytes)    Red 4 (3).JPG (380593 bytes)   Red 6.JPG (371506 bytes)   Red 6 (2).JPG (358190 bytes)   
            4                4              6               6
*notice the piece of chewed leaf in #4's close-up 

 Red 8.JPG (414167 bytes)    Red 8 (1).JPG (324298 bytes)
   
     8                8


Emerged on Sept. 24,2005   Lab Pics Sept. 24 (27).JPG (359535 bytes)

                Black Group (Blocks 2A & 4B)

Lab Pics Sept. 24 (20).JPG (365134 bytes)  Lab Pics Sept. 24 (23).JPG (383606 bytes)  Lab Pics Sept. 24 (17).JPG (406597 bytes)  Lab Pics Sept. 24 (18).JPG (350885 bytes)   
      2                3                8               8

                Blue Group (Blocks 3A & 4B)

Lab Pics Sept. 24 (12).JPG (406135 bytes)  Lab Pics Sept. 24 (13).JPG (359002 bytes)
     3                3


Did not Emerged

Lab Pics Sept. 24 (28).JPG (338897 bytes)    Lab Pics Sept. 24 (4).JPG (307991 bytes)
      7, 5, 2            2
* Were replaced with a small larvae that had already hatched.


* Because I began taking pictures to capture what the students were not able to see (outside of class time), I did not take any pictures in the interim between the larvae hatching and the adults emerging from their pupal stages.  Here are a few pictures of swallowtail caterpillars I have taken over the years.  On their data sheets, the students do have drawings that document the growth of the caterpillars until they entered the pupal stage (chrysalis).  

 research plants aug. 19 (7).JPG (391751 bytes)   research plants aug. 19 (1).JPG (367659 bytes)  backyard Aug 19 (4).JPG (358327 bytes)   W9 045.jpg (362046 bytes)   MVC-070S.JPG (37097 bytes)
 W9 048.jpg (363212 bytes)   Nana's Aug. 18 (8).JPG (372107 bytes) Nana's yard Aug. 20.JPG (389068 bytes) MVC-058S.JPG (37328 bytes)
  
Look at those                                        What they look like right 
    chew marks!                                          before forming pupa (chrysalis)


#A2 from my Research Project
Sept. 17.JPG (400746 bytes)
Sept. 20 (9).JPG (404912 bytes)  Sept. 21 (17).JPG (406158 bytes)   W9 029.jpg (391353 bytes)   
        Sept.17                 Sept. 20                Sept. 21             Sept. 23

 Sept. 25 (14).JPG (371163 bytes)    Sept. 26 (12).JPG (371144 bytes)    Inside A2 Oct. 02 (3).JPG (365500 bytes)
        Sept. 25                  Sept. 26                 Oct. 02

 

Ovipositing (egg laying) female
Ovipositing female (8).JPG (360488 bytes)



IT'S A...  Black Swallowtail Butterfly!


Eclosed (emerged from Chrysalis) on Oct. 20, 2005


     
        7                 7


IT'S A...  Black Swallowtail Butterfly!

Eclosed (emerged from Chrysalis) on Oct. 22, 2005

red 7 Oct. 22 (1).JPG (367522 bytes)    red 7 Oct. 22 (4).JPG (379144 bytes)    red 7 Oct. 22 (9).JPG (364193 bytes)     
                      7 ready to emerge               

RED.JPG (377604 bytes)   Red 7 emerging Oct. 22 (1).JPG (411802 bytes)   Before filling wings.JPG (396973 bytes)
         7 EMERGING!             7 before
                                  spreading wings
 

 Starting to fill wings.JPG (363925 bytes)    Filling Wings.JPG (354826 bytes)    Adult.JPG (397142 bytes)
          7 filling wings             Adult  7                  
                          

#7 was not photographed as an egg because it had already
emerged when the students adopted it for study.
  


IT'S A...  Black Swallowtail Butterfly!

Eclosed (emerged from Chrysalis) on Oct. 22 or 23, 2005

Black.JPG (388217 bytes)

           


IT'S A...  Black Swallowtail Butterfly!

Eclosed (emerged from Chrysalis) on Oct. 25 or 26, 2005

six.JPG (384058 bytes)
          6


IT'S A...  Black Swallowtail Butterfly!

Eclosed (emerged from Chrysalis) on Oct. 26, 2005

thirteen c.JPG (403642 bytes)    thirteen a.JPG (388787 bytes)
            3                3
 
right before
     emerging
   


Oct. 26 Red 8 (21).JPG (378292 bytes)    Oct. 26 Red 8 (29).JPG (424240 bytes)    Oct. 26 Red 8 (31).JPG (427534 bytes)
         8                 8                 8
filling wings                                 shows colors
  with blood                                      nicely


blue.JPG (336267 bytes)
       3  

IT'S A...  Black Swallowtail Butterfly!

Eclosed (emerged from Chrysalis) on Oct. 27, 2005

Red 6 Oct. 27 (12).JPG (398259 bytes)
           

 

 

LINK TO GOOD WEBSITE FOR INFO ON BLACK SWALLOWTAILS!

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This site is maintained by Mrs. Lant, for her student's, at F. W. Cox HS.  Please e-mail any comments or suggestions!
Last modified: May 31, 2008
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